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Morphological changes in the rat aorta endothelium at the clamping sites

Lazar Jelev, Roman Romansky, Kouzman Guirov, Minko Minkov, Wladimir Ovtscharoff


This study aimed to investigate on en face preparations the morphological changes in the rat aorta endothelium at the clamping sites while performing surgical anastomosis.

Adult male Wistar rats (14-18-month-old, 390-420g) were used for the experiments. Under surgical anesthesia, the postrenal part of the abdominal aorta was dissected, clamped proximally and distally, cut and restored microsurgically using 10-0 suture. The clamping time was 30-40 min. At different days after surgery the animals were sacrificed, their aortas fixed and removed from the body and preparations for en face observation were made.

En face preparations, obtained at the first postoperative day, showed complete denudation of the clamping sites. The most interesting endothelial cell population was noted at the borders of the clamping sites on the 3rd day after surgery - a large number of small endothelial cells and also few endothelial cells having very large size. At the later stages, the endothelial cell layer advanced rapidly to the denuded areas, as the complete restoration was observed after day 14.

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Becquemin JP, Deleuze P, Léandri J, Lautier

A, Mellière D. Influence of the type of arterial

anastomosis on the development of endothelial

hyperplasia. - J Mal Vasc. 9, 1984, No 2, 90-94.

Bunt TJ, Manship L, Moore W. Iatrogenic

vascular injury during peripheral

revascularization. - J Vasc Surg. 2, 1985, No 3,


Coelho JC, Sigel B, Flanigan DP, Schuler JJ,

Justin J, Machi J. Arteriographic and ultrasonic

evaluation of vascular clamp injuries using an in

vitro human experimental model. - Surg Gynecol

Obstet. 155, 1982, No 4, 506-512.

Folts J. An in vivo model of experimental

arterial stenosis, intimal damage, and periodic

thrombosis. - Circulation. 83, 1991, No 6, IV3-14.

Jelev L, Romansky R, Nachev G, Ovtscharoff W.

Regeneration of the endothelium of the rat aorta

after microsurgical anastomosis studied on en

face preparations. - Comptes rendus de l`Académie

bulgare des Sciences 62, 2009, No 12, 1621-1624.

Jelev L, Surchev L. A novel simple technique for

en face endothelial observations using watersoluble

media -`thinned-wall` preparations. - J

Anat. 212, 2008, No 2, 192-197.

Jones GT, Martin BJ, Stehbens WE. Endothelium

and elastic tears in the afferent arteries of

experimental arteriovenous fistulae in rabbits. -

Int J Exp Pathol. 73, 1992, No 4, 405-416.

Mattox DE. Microvascular anastomosis:

a scanning electron microscopic study. -

Otolaryngol Head Neck Surg, 88, 1980, 252-256.

Pabst TS 3rd, Flanigan DP, Buchbinder D.Reduced

intimal injury to canine arteries with controlled

application of vessel loops. - J Surg Res., 47, 1989,

No 3, 235-241.

Slayback JB, Bowen WW, Hinshaw DB. Intimal

injury from arterial clamps. - Am J Surg. 132,

, No 2, 183-188.

Wieslander JB, Mecklenburg CV, Aberg M.

Endothelialization following end-to-end and

end-in-end (sleeve) microarterial anastomoses.

A scanning electron microscopic study. - Scand J

Plast Reconstr Surg, 18, 1984, 193-199.

Zeebregts C, van den Dungen J, Buikema H, van

der Want J, van Schilfgaarde R. Preservation

of endothelial integrity and function in

experimental vascular anastomosis with nonpenetrating

clips. - Br J Surg. 88, 2001, No 9,


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About The Authors

Lazar Jelev
Medical University of Sofia

Department of Anatomy, Histology and Embryology

Roman Romansky
Alexandrovska university hospital of Sofia

Department of plastic and reconstructive surgery

Kouzman Guirov
Military medical academy

Clinic of vascular surgery and angiology

Minko Minkov
Medical university of Varna

Department of anatomy, histology and embryology

Wladimir Ovtscharoff
Medical university of Sofia

Department of anatomy, histology and embryology

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