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Gastroprotective effect of ethanolinfusi on from Cotinus coggygria wood in rats

Danail Pavlov, Miroslav Eftimov, Maria Tzaneva, Diana Ivanova, Milka Nashar, Ina Kobakova, Stefka Valcheva-Kuzmanova


Purpose: The Smoke tree (Cotinus coggygria) is a medicinal plant that is traditionally used by the Balkan and Anatolian folk medicine for its antiseptic and anti-inflammatory properties. There are few reports about the internal usage of ethanol extracts from C. coggygria wood against gastric ulcer. The aim of this study was to explore the effect of ethanol infusion from Cotinus coggygria wood (EICCW) on indomethacin- induced ulcerogenesis in rats and its possible effect on the gastric oxidative status.

Material and Methods: EICCW was applied by oral gavage (volume: 10 ml/kg) as a pretreatment 3 days before a single intragastric administration of indomethacin (dose: 100 mg/kg). Gastric erosions were evaluated histopathologically. Malondialdehyde (MDA) in blood serum and stomach was measured as a biochemical marker of lipid peroxidation. Gastric necrosis was also evaluated by alkaline phosphatase (ALP) and uric acid (UA) assays.

Results: EICCW reduced the elevated by indomethacin gastric MDA, ALP and UA levels. Histopathological studies demonstrated that EICCW induced a reduction of the depth and severity of indomethacin-induced mucosal lesions.

Conclusion: Indomethacin-induced gastric mucosal damage was accompanied by the development of oxidative stress. EICCW-pretreatment alleviated the gastric lesions, and prevented the indomethacin-induced elevation of gastric ALP and UA. It could be suggested that the gastroprotective effect of EICCW was due to its antioxidant properties as evidenced by the decreased gastric MDA levels.


Cotinus coggygria; Indomethacin; Gastric mucosal damage; Oxidative stress; Lipid peroxidation; Antioxidant activity

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About The Authors

Danail Pavlov
Medical University of Varna

Department of Biochemistry, Molecular Medicine and Nutrigenomics

Miroslav Eftimov
Medical University of Varna

Department of Preclinical and Clinical Pharmacology

Maria Tzaneva
Medical University of Varna

Department of Preclinical and Clinical Sciences

Diana Ivanova
Medical University of Varna

Department of Biochemistry, Molecular Medicine and Nutrigenomics

Milka Nashar
Medical University of Varna

Department of Biochemistry, Molecular Medicine and Nutrigenomics

Ina Kobakova
Medical University of Varna

Department of General and Clinical Pathology, Forensic Medicine and Deontology, Medical University of Varna

Stefka Valcheva-Kuzmanova
Medical University of Varna

Department of Preclinical and Clinical Pharmacology

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