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Effects of mulberry heartwood extract on genes related to lipid metabolism

Milena Pasheva, Milka Nashar, Oskan Tasinov, Diana Ivanova


Scientific evidence exists that extracts from different parts of Morus nigra (mulberry tree), mainly fruits, leaves and root bark, exhibit various beneficial effects, such as antiviral, antihyperglycemic, antiatherogenic and hypotensive activity. Although it is known that the heartwood has a specific phytochemical composition, the biological effects of this part of the plant are not yet clarified. This study examines the effect of 40% ethanol infusion from mulberry heartwood on the expression of three genes related to lipid metabolism: CCAAT/enhancer binding protein-alpha (C/EBPα) gene, adipogenic transcriptional factors peroxisome proliferator-activated receptor gamma (PPARγ) and fatty acid-binding protein (aP2) genes in adipocytes. Results obtained present that treatment with two concentrations of mulberry ethanol extract decreased expression of aP2 messenger RNA as compared to controls. Based on these results we concluded that heartwood mulberry extract had beneficial effects on lipid metabolism and could be a potential source for search of active compounds for treatment of metabolic disorders related to adipose tissue metabolism.


morus nigra; heartwood extract; adipocytes; gene expression; lipid metabolism

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Barati S, Momtaze H, Azhdary mamoreh M. The effect of hydro-alcoholic extract of Morus nigra leaf on lipids and sugar in serum of diabetic rats. Asian J Biomed Pharm Sci. 2012;2(15):38-40.

Beaudoin A. New technique for revealing latent fingerprints on wet, porous surfaces: Oil Red O. J Forensic Identif. 2004;54 (4):413-421.

Bordoni A, Di Nunzio M, Danesi F and Biagi PL. Polyunsaturated fatty acids: from diet to binding To PPARs and other nuclear receptors. Genes & Nutrition. 2006;1(2):95-106.

Chan KC, Ho HH, Lin MC, Wu CH, Huang CN, Chang WC, et al. Mulberry water extracts inhibit rabbit atherosclerosis through stimulation of vascular smooth muscle cell apoptosis via activating p53 and regulating both intrinsic and extrinsic pathways. J Agric Food Chem. 2014;62(22):5092-5101.

Chan KC, Yang MY, Lin MC, Lee YJ, Chang WC, Wang CJ. Mulberry leaf extract inhibits the development of atherosclerosis in cholesterol- fed rabbits and in cultured aortic vascular smooth muscle cells. J Agric Food Chem. 2013;61(11):2780-2788.

Choi BH, Ahn IS, Kim YH, Park JW, Lee SY, Hyun CK, Do MS Berberine reduces the expression of adipogenic enzymes and inflammatory molecules of 3T3-L1 adipocyte. Exp Mol Med. 2006;38:599-605.

Cristancho AG, Lazar MA. Forming functional fat: a growing understanding of adipocyte differentiation. Nat Rev Mol Cell Biol. 2011;28:722-34.

De Rosso MD, Cancian D, Panighe, A, Vedova AD, Flamini R. Chemical compounds released from five different woods used to make barrels for aging wines and spirits: volatile compounds and polyphenols. Wood Sci Tech. 2009;43:375-385.

DeFuria J, Bennett G, Strissel KJ, Perfield JW 2nd, Milbury PE, Greenberg AS, et al.. Dietary blueberry attenuates whole-body insulin resistance in high fat-fed mice by reducing adipocyte death and its inflammatory sequelae. J Nut. 2009;139:1510-1516.

Ejaz A, Wu D, Kwan P, Meydani M,. Curcumin inhibits adipogenesis in 3T3-L1 adipocytes and angiogenesis and obesity in C57/BL mice. J Nutr. 2009;139:919-925.

Flamini R, Vedova AD, Cancian D, Panighel A, de Rosso M. GC/MS-positive ion chemical ionization and MS/MS study of volatile benzene compounds in five different woods used in barrel making. J Mass Spectrom. 2007;42:641-646.

Habauzit V. and Morand C. Evidence for a protective effect of polyphenols-containing foods on cardiovascular health: an update for clinicians. Ther Adv Chronic Dis. 2012 Mar;3(2):87-106.

Hanhoff T, Lucke C and Spener F. Insights into binding of fatty acids by fatty acid binding proteins. Molecular and Cellular Biochemistry. 2002;239:45-54.

Hsieh YH, Wang SY. Lucidone from Lindera erythrocarpa Makino fruits suppresses adipogenesis in 3T3-L1 cells and attenuates obesity and consequent metabolic disorders in high-fat diet C57BL/6 mice. Phytomedicine. 2013;20(5):394-400.

Hu X, Wu JW, Zhang XD, Zhao QS, Huang JM, Wang HY, Hou AJ. Isoprenylated flavonoids and adipogenesis-promoting constituents from Morus nigra. J Nat Prod. 2011;74(4):816-24.

Huang C, Zhang Y, Gong Z, Sheng X, Li Z, Zhang W, Qin Y. Berberine inhibits 3T3-L1 adipocyte differentiation through the PPARgamma pathway. Biochem Bioph Res Com. 2006;348:571-578.

Ivanova D, Vankova D, Nashar M. Agrimonia eupatoria tea consumption in relation to markers of inflammation, oxidative status and lipid metabolism in healthy subjects. Arch Physiol Biochem. 2013;119(1):32-7.

Jeon T, Hwang SG, Hirai S, Matsui T, Yano H, et al. Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells. Life Sci. 2004;12:3195-3203.

Kolehmainen M, Vidal H, Alhava E, Uusitupa MI. Sterol regulatory element binding protein 1c (SREBP-1c) expression in human obesity. Obes Res. 2001;9(11):706-12.

Kumar A, Chauhan PK, Bhardwaj VS, Kumar R, Tyagi A. In vitro antioxidant and phytochemical investigations of ethanolic extracts of Viola serpens and Morus nigra. J Chem Pharm Res. 2011;3(4):166-171.

Lin J, Della-Fera MA, Baile CA. Green tea polyphenol epigallocatechin gallate inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes. Obesity Res. 2005;13: 982-990.

Liu LK, Chou FP, Chen YC, Chyau CC, Ho HH, Wang CJ. Effects of mulberry (Morus alba L.) extracts on lipid homeostasis in vitro and in vivo. J Agric Food Chem. 2009;57(16):7605-7611.

Liu, LK, Lee HJ, Shih YW, Chyau CC, Wang CJ Mulberry anthocyanin extracts inhibit LDL oxidation and macrophage-derived foam cell formation induced by oxidative LDL. J Food Sci. 2008;73:H113-H121.

Livak, K. J., Schmittgen T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔC(t) method. Methods 2001;25(4):402-408.

Mahmoud M. Y. Natural antioxidants effect of mulberry fruits (Morus nigra and Morus alba L.) on lipids profile and oxidative stress in hypercholestrolemic rats. Pakistan J Nutr. 2013;12:665-672.

Pandey KB and Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2009;2(5):270-278.

Pasheva M, Pavlov D, Tasinov O, Nashar M, Kiselova-Kaneva Y, Ivanova D. Cytoprotective and cytoproliferative effects of ethanol infusions from Morus nigra and Cotinus coggygria heartwood in 3T3-L1 preadipocytes. Scripta Scientifica Medica. 2013;45 Suppl 6:25-28.

Pasheva М, Nashar M, Pavlov D, Slavova S, Ivanov D, Ivanova D. Antioxidant capacity of different woods traditionally used for coloring hard alcoholic beverages in Bulgaria. J Sci Technol. 2013;3(1):123-127.

Patel DK, Prasad SK, Kumar R, Hemalatha S. An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed. 2012; 2(4):320-330.

Pawlowska AM, Oleszek W, Braca A Quali-quantitative analyses of flavonoids of Morus nigra L. and Morus alba L. (Moraceae) fruits. J Agric Food Chem. 2008;56:3377-3380.

Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM Transcriptional regulation of adipogenesis. Genes Dev. 2000;14:1293-1307.

Song Y, Park HJ, Kang SN, Jang SH, Lee SJ, Ko YG, et al. Blueberry peel extracts inhibit adipogenesis in 3T3-L1 cells and reduce high-fat diet-induced obesity. PLoS One. 2013;8(7):e69925.

Venkatesh KR, Chauhan S. Mulberry: Life enhancer. J Med Plants Res. 2008;2(10):271-278.

Volpato GT, Calderona IMP, Sinzato S, Campos KE, Rudge MVC, Damasceno DC. Effect of Morus nigra aqueous extract treatment on the maternal-fetal outcome, oxidative stress status and lipid profile of streptozotocin-induced diabetic rats. J Ethnopharm. 2011;138(3):691-696.

Xie, W., Gu, D., Li, J., Cui, K., Zhang, Y.,. Effects and action mechanisms of berberine and Rhizoma coptidis on gut microbes and obesity in high-fat diet-fed C57BL/6J mice. PLoS ONE 2011;6:e24520.

Zheng ZP, Cheng KW, Zhu Q, Wang XC, Lin ZX, Wang M. Tyrosinase inhibitory constituents from the roots of Morus nigra: A structure−activity relationship study. J Agric Food Chem. 2010;58(9):5368-5373.



About The Authors

Milena Pasheva
Medical university of Varna

Department of Biochemistry, Molecular Medicine and Nutrigenomics

Milka Nashar
Medical university of Varna

Department of Biochemistry, Molecular Medicine and Nutrigenomics

Oskan Tasinov
Medical university of Varna

Department of Biochemistry, Molecular Medicine and Nutrigenomics

Diana Ivanova
Medical university of Varna

Department of Biochemistry, Molecular Medicine and Nutrigenomics

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