Commercial tea comes from plants belonging to a relatively large group of cultivated species of Camellia sinensis (L) O. Kuntze. It is one of the oldest known beverages made from the tender leaves of the plants. The main aim of the present study is to analyze the presence of biochemicals in UPASI -16 clone. In the results, the UPASI -16 clone showed high catechin (22.88%), polyphenol (31.70%), polyphenol oxidase (1178.63 U/mg of protein) and tea enzyme peroxidase (729.72 µM of O2 formed min-1g-1 dry weight), catalase (1.89 µM H2O2 reduced min-1 mg-1 protein) and Super oxide dismutase (58.45 U/mg proteins). UPASI -16 showed high catechin content in segregated tea crop shoots from the first internodes (23.66%) followed by the first leaf (22.46%), second leaf and third leaf. Tea quality flavonoid gene expression, while ANR (1.66%) and F3H (1.02%) were down regulated, F35H and ANS (2.82%) were up regulated in UPASI -16. Based on the results, it can be concluded that crop shoots of Camellia sinensis (L) O. Kuntze selected clone of UPASI-16 may possess high amount of biochemicals and may further lead to development of commercial tea.
Yamamoto T, Juneja LR, Chu DC, Kim M. (Eds). Chemistry and Application of Green Tea. New York: CRC Press; 1998.
Rogers PJ, Smith JE, Heatherley SV, Pleydell-Pearce CW. Time for tea: mood, blood pressure and cognitive performance effects of caffeine and theanine administered alone and together. Psychopharmacology. 2008;195:569-577.
Park JS, Kim JB, Hahn BS, Kim KH, Ha SH, Kim YH. EST analysis of genes involved in secondary metabolism in Camellia sinensis (tea), using suppression subtractive hybridization. Plant Sci. 2004;166:953-961.
Chen L, Zhao LP, Gao QK. Generation and analysis of expressed sequence tags from the tender shoots cDNA library of tea plant (Camellia sinensis). Plant Sci. 2005;168:359-363.
Richelle M, Tavazzi I, Offord E. Comparison of the antioxidant activity of commonly consumed polyphenolic beverages (coffee, cocoa, and tea) prepared per cup serving. J Agric Food Chem. 2001;49:3438-3442.
Starlin T, Ragavendran P, Raj CA, Perumal PC, Gopalakrishnan VK. Element and functional group analysis of Ichnocarpus frutescens R. Br. (Apocynaceae). Int J Pharm Pharm Sci. 2012;4:343-345.
Perumal PC, Sophia D, Raj CA, Ragavendran P, Starlin T, Gopalakrishnan VK. In vitro antioxidant activities and HPTLC analysis of ethanolic extract of Cayratia trifolia (L.). Asian Pac J Trop Dis. 2012;2:S952-S956.
Poornima K, Perumal PC, Gopalakrishnan VK. Protective effect of ethanolic extract of Tabernaemontana divaricata (L.) R. Br. against DEN and Fe NTA induced liver necrosis in Wistar Albino rats. Biomed Res Int. 2014;2014:240243.
Perumal PC, Sowmya S, Pratibha P, Vidya B, Anusooriya P, Starlin T, et al., Identification of novel PPARγ agonist from GC-MS analysis of ethanolic extract of Cayratia trifolia (L.): a computational molecular simulation studies. J App Pharm Sci. 2014;4:006-011.
Pratibha P, Sophia D, Perumal PC, Gopalakrishnan VK. In-silico docking analysis of Emilia sonchifolia (l.) dc. gas chromatography-mass spectroscopy derived terpenoid compounds against pancreatic cancer targets (AKT and BRCA2). World J Pharm Pharm Sci. 2014;3:1844-1855.
Perumal PC, Sowmya S, Pratibha P, Vidya B, Anusooriya P, Starlin T, et al. Isolation, structural characterization and in silico drug-like properties prediction of bioactive compound from ethanolic extract of Cayratia trifolia (L.). Pharmacog Res. 2015;7:121-125.
Cabrera C, Artacho R, Gimenez R. Beneficial effects of green tea - a review. J Am Col Nutr. 2006;25:79-99.
Topalova T, Jelev I, Georgieva S. Quantitative determination of methylxanthines and polyphenols in plant substances. Manage Educat. 2013;9(5):110-114.
Ramkumar S, Sureshkumar P, Mandal AKA, Rajaram K, Mohankumar P. Identification of superior varieties of tea (Camellia sinensis (l.) o. kuntze) in the selected UPASI germplasm using biomarkers. Int J Phys Sci. 2011;6(5):727-743.
Rawat R, Gulati A. Seasonal and clonal variations in some major glycosidic bound volatiles in Kangra tea (Camellia sinensis (L.) O. Kuntze). European Food Res Technol. 2008;226(6):1241-1249.
Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of proteins using the principle of protein-dye -binding. Analytical Biochem. 1976;72:248-254.
Dev-Choudhury MN, Goswami MR. A rapid method for the estimation of total polyphenolic matters in tea Camellia sinensis L. Two Bud. 1983;30:59-61.
Swain T, Hillis WE. The phenolic constituents of Prunus domestica L.-The quantitative analysis of phenolic constituents. J Sci Food Agric. 1959;10:63-68.
Chance B, Maehly AC. Assay of catalase and peroxidases. Methods Enzymol. 1955;2:764-775.
Luck H. In: methods in enzymatic analysis. 2nd Ed. New York: Bergmeyer Academic Press; 1974.
Van-Rossun MWPC, Alberda M, Van-Derplas LHW. Role of oxidative damage in tulip bulb scale micro propagation. Plant Sci. 1997;130:207-221.
Jain, Arun K, Acito F, Malhotra NK, Mahajan V. A Comparison of the Internal Validity of Alternative Parameter Estimation Methods in Decompositional Multiattribute Preference Models. J Mark Res. 1979;16:313-322.
Gomez KA, Gomez AA. Statistical procedures for agricultural research with emphasis of rice. Int Rice Res Ins, Los Banos, Philippines; 1976.
Sealy J. Species of Camellia in cultivation. R Hort Soc. 1937;62:352-369.
Satyanarayana N, Sharma VS. UPASI Biclonal Seed Stocks, Proceedings of the Sixth Joint Area Scientific Symposium (JASSVI). UPASI Tea Res Ins Bulletin. 1993;46:144-154.
Balasaravanan T, Pius PK, Kumar RR, Muraleedharan N, Shasany AK. Genetic diversity among south Indian tea germplasm (Camellia sinensis, C. assamica and C. assamica spp. lasiocalyx) using AFLP markers. Plant Sci. 2003;165:365-372.
Jayaganesh S, Venkatesan S. Impact of Magnesium Sulphate on Biochemical and Quality Constituents of Black Tea. American J Food Tech. 2010;5:31-39.
Collakova E, Klumas C, Suren H, Myers E, Heath LS, Holliday JA, et al. Evidence for extensive heterotrophic metabolism, antioxidant action and associated regulatory events during winter hardening in Sitka spruce. BMC Plant Biol. 2013;13(72):1-16.
Kerdnaimongkol K, Woodson WR. Inhibition of Catalase by Antisense RNA Increases Susceptibility to Oxidative Stress and Chilling Injury in Transgenic Tomato Plants. J American Soc Hort Sci. 1999;124(4):330-336.
Punyasiri P, Abeysinghe IS, Kumar V, Treutter D, Duy D, Gosch C, et al. Flavonoid biosynthesis in the tea plant Camellia sinensis: properties of enzymes of the prominent epicatechin and catechin pathways. Arch Biochem Biophys. 2004;431:22-30.