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Phenotype of De Novo Generated Cells in the Spinal Cord of Adult Macaque Monkeys

Dessislava Marinova, Meglena Angelova, Vesselina Mihaleva, Stoyan Pavlov, Vanya Goranova, Tetsumori Yamashima, Anton Tonchev

Abstract

Neuronal stem and progenitor cells exist in the spinal cord of sexually mature mammals. Òhåy play an important role during repaining processes after in- jury, but their proliferation and differentiation are limited. In the present study we used the proliferative marker bromodeoxyuridine (BrdU) for a short (2 h) and three longer survival periods (2, 5 and 10 weeks) to investigate the quantity, topography and fate of de novo generated cells in intact spinal cord of adult pri- mates (macaque monkeys). We applied as well single or in combinations markers for mesenchymal cells or/ and neuronal stem/progenitor cells to demonstrate the phenotype of the proliferating cells. We found that af- ter the short period of BrdU application (2 h) the num- ber of BrdU+ cells is significantly elevated only in the cervical segments. Most of the cells in the ependymal layer are immunopositive for Vimentin or/and Nestin. This is an indice for their cellular belonging. A con- siderable number of Vimentin+ cells of the ependy- mal layer form long characteristic processes directed to underlying blood capillaries. Òhis indicates their participation as a component of the ependymal cellu- lar niche. The presence of BrdU+/Nestin+ cells in the central canal surrounding zone confirms the existence of dividing neuronal stem/progenitor cells cells in the spinal cord of adult primates.

Keywords

macaque; spinal cord; progenitors; BrdU; Vimentin; Nestin

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DOI: http://dx.doi.org/10.14748/vmf.v3i1.1108

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