Introduction
Knowledge in the field of microribonucleic acids (miRNAs) can be used both to reveal the mechanisms of occurrence of inflammatory bowel diseases (IBDs) and to predict the response to a particular treatment.
Aim
The aim of this article is to assess the serum expression of miR-1228 in IBD patients.
Materials and Methods
A total of 35 patients with ulcerative colitis (UC), 35 patients with Crohn’s disease (CD), and a control group of 30 healthy individuals were studied. Serum expression of miR-1228 was tested in all through reverse transcriptase-quantitative real-time polymerase chain reaction (RT-qPCR).
Results
The expression in CD patients (1.91 ±2.21) was significantly higher compared to the expression in UC patients (1.15±0.64) and healthy controls (1.14±0.59). In CD patients, increased expression of miR-1228 was observed in cases with active disease, intestinal localization (L1–2.28) and penetrating phenotype (B3–3.92). In UC patients, no difference in miR-1228 expression was observed with regards to the localization and the course of the disease. Serum expression of miR-1228 correlates positively with CRP in UC patients.
Conclusion
In the present study, increased expression of miR-1228 was observed in cases with active disease (UC, CD), intestinal localization (CD), and penetrating phenotype (CD), while decreased expression correlated to immunosuppressive (CD) and biological (UC) therapy.
Ananthakrishnan AN, Cheng SC, Cai T, et al. Association between reduced plasma 25-hydroxy vitamin D and increased risk of cancer in patients with inflammatory bowel diseases. Clin Gastroenterol Hepatol. 2014;12:821–827
Berezikov E, Chung WJ, Willis J, Cuppen E, Lai EC. Mammalian mirtron genes. Mol Cell 2007;28:328–36
Guo, H.; Ingolia, N.T.;Weissman, J.S.; Bartel, D.P. Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature 2010, 466, 835–840
Ham M, Longhi MS, Lahiff C, Cheifetz A, Robson S, Moss AC. Vitamin d levels in adults with Crohn’s disease are responsive to disease activity and treatment. Inflamm Bowel Dis. 2014;20:856–860
Kong R, Luo H, Wang N, et al. Portulaca Extract Attenuates Development of Dextran Sulfate Sodium Induced Colitis in Mice through Activation of PPARgamma. PPAR Res 2018;2018:6079101
Mulligan ML, Felton SK, Riek AE, Bernal-Mizrachi C. Implications of vitamin D deficiency in pregnancy and lactation. Am J Obstet Gynecol 2010;202(429):e421–9
Willson TM, Brown PJ, Sternbach DD, et al. The PPARs:from orphan receptors to drug discovery. J Med Chem 2000;43:527-50
Yang L, Bian Y, Li Z, YanY , Li J , Li W , Li Zeng. Identification of potential biomarkers and pathways in ulcerative colitis with combined public mRNA and miRNA expression microarray data analysis. J Gastrointest Oncol 2019;10(5):847-858. http://dx.doi.org/10.21037/jgo.2019.06.06
Yao J, Lu Y, Zhi M, et al. Dietary n3 polyunsaturated fatty acids ameliorate Crohn’s disease in rats by modulating the expression of PPARgamma/NFAT. Mol Med Rep 2017;16:8315-22
Zhao, H., Pullagura, S. R. N., Rieger, S., & Lisse, T. S. Vitamin D and MicroRNAs. Vitamin D,2018, 245–267. doi:10.1016/b978-0-12-809965-0.00015-x
