Obesity is a disorder of high prevalence and social significance. Anethole is an aromatic compound found in anise and fennel essential oils. It produces antioxidant, anti-inflammatory and other beneficial effects.
The aim of this study was to evaluate the effect of anethole, administered in increasing concentrations, on visceral adiposity and serum lipids in rats on a high-calorie diet (HCD).
Fifty male Wistar rats were allocated into 5 groups: control, HCD, HCD+62.5A, HCD+125A, and HCD+250A. The HCD groups received a high-fat high-fructose diet and 10% fructose solution, and the control group received a standard laboratory diet and tap water. The HCD+62.5A, HCD+125A, and HCD+250A groups were treated daily orally with anethole dissolved in sunflower oil in a volume of 3 mL/kg at doses of 62.5 mg/kg, 125 mg/kg, and 250 mg/kg, respectively. The control and HCD groups received the solvent in the same volume and route of administration. The duration of the experiment was 10 weeks. At its end, blood samples were taken for biochemical tests and visceral adipose tissue was weighed. The results were analyzed by one-way ANOVA followed by Dunnett's post-test with the statistical program GraphPad Prism 5.0.
The visceral obesity index was significantly increased (p<0.05) in all groups receiving HCD except for HCD+250A. There was no significant increase in plasma triglycerides and cholesterol in the HCD group compared to the control one. Anethole treatment did not change significantly the triglyceride and cholesterol levels compared to these of the HCD group.
Anethole treatment affected beneficially the development of visceral obesity and did not change significantly serum triglyceride and cholesterol levels in rats receiving a high-calorie diet.
Afiat M, Amini E, Ghazanfarpour M, Nouri B, Mousavi MS, Babakhanian M, Rakhshandeh H. The Effect of Short-term Treatment with Fennel on Lipid Profile in Postmenopausal Women: A Randomized Controlled Trial. Journal of menopausal medicine, 24(1), 2018; 29–33.
Ahmed H. Kissebah, David S. Freedman, Alan N. Peiris. Health Risks of Obesity. Medical Clinics of North America,Volume 73, 1,1989; 111-138
Bae, JiYoung et al. Fennel (Foeniculum vulgare) and Fenugreek (Trigonella foenum-graecum) Tea Drinking Suppresses Subjective Short-term Appetite in Overweight Women.. Clinical nutrition research vol. 4,3. 2015; 168-74.
Bandyopadhyay, Dhrubajyoti et al. Safety and Efficacy of Extremely Low LDL-Cholesterol Levels and Its Prospects in Hyperlipidemia Management. Journal of lipids vol. 2018;8598054.
Caiyun Yu, Jiaqi Zhang, Qiming Li, Xiaoe Xiang, Zaibin Yang, Tian Wang,
Effects of trans-anethole supplementation on serum lipid metabolism parameters, carcass characteristics, meat quality, fatty acid and amino acid profiles of breast muscle in broiler chickens. Poultry Science. 2021;101484
Gancheva S, Zhelyazkova-Savova M, Galunska B, Chervenkov T. Experimental models of metabolic syndrome in rats. Scripta Scientifica Medica, 2015, 47(2), 14-21.
Helal E, Abdelaziz M, Ahmed SS. Effect of Anise (Pimpinella Anisum L.) as Phytoestrogen on Some Sex Hormones and Biochemical Parameters. The Egyptian Journal of Hospital Medicine, 2019, 75; 1918-1922.
Hur MH, Kim C, Kim CH, Ahn HC, Ahn HY. The effects of inhalation of essential oils on the body weight, food efficiency rate and serum leptin of growing SD rats. Taehan Kanho Hakhoe Chi. 2006 Apr; 36(2):236-43.
Kang NH, Mukherjee S, Min T, Kang SC, Yun JW. Trans-anethole ameliorates obesity via induction of browning in white adipocytes and activation of brown adipocytes. Biochimie. 2018;151:1-13
Kang P, Kim KY, Lee HS, Min SS, Seol GH. Anti-inflammatory effects of anethole in lipopolysaccharide-induced acute lung injury in mice. Life Sci. 2013;93(24):955-61
Kim SJ, Kim KS, Shin SU, Choi YM, Kang BG, Yoon YS et al. A clinical study of decrease appetite effects by aromatherapy using foeniculum vulgare mill (fennel) to female obese patients. J Korean Orient Assoc Stud Obes. 2005;5:9–20.
Marinov V, Vulcheva-Kuzmanova S. Review on the pharmacological activities of anethole. Scripta Scientifica Pharmaceutica, [S.l.], v. 2, n. 2, 2015; 14-19
Marseglia L, Manti S, D'Angelo G, Nicotera A, Parisi E, Di Rosa G, Gitto E, Arrigo T. Oxidative stress in obesity: a critical component in human diseases. Int J Mol Sci. 2014, 26;16(1):378-400
Nawal A. Elghazaly, Eman H. Radwan, Hala H. Zaatout, Mohamed M. Elghazaly, Nour El din Allam. Beneficial Effects of Fennel (Foeniculum Vulgare) in Treating Obesity in Rats. Journal of Obesity Management . 2019; 1(2):16-33.
Ponte EL, Sousa PL, Rocha MV, Soares PM, Coelho-de-Souza AN, Leal-Cardoso JH, Assreuy AM. Comparative study of the anti-edematogenic effects of anethole and estragole. Pharmacol Rep. 2012;64(4):984-90
Samadi-Noshahr Z, Hadjzadeh M-A, Moradi-Marjaneh R, Khajavi-Rad A. The hepatoprotective effects of fennel seeds extract and trans-Anethole in streptozotocin-induced liver injury in rats. Food Sci Nutr. 2021; 9: 1121– 1131.
Song A, Park Y, Kim B, Lee SG. Modulation of Lipid Metabolism by Trans-Anethole in Hepatocytes. Molecules. 2020;25(21):4946
Walker S.Carlos Poston, John P. Foreyt,Obesity is an environmental issue,Atherosclerosis,Volume 146, 2,1999;201-209
Wright, SM, Aronne LJ. Causes of obesity. Abdom Radiol 37, 2012; 730–732
