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Microorganisms colonizing tooth structures and antibacterial effect of temporary filling materials

Boris Valkov, Miglena B Balcheva, Neli Ermenlieva


The oral cavity contains a variety of microorganisms. Some of them are pathogens. Various groups of microorganisms play roles in causing different diseases affecting the hard dental tissues, the pulp, and the periodontium. The microflora found in dentin, the pulp chamber, and the root canals closely resembles that present in the oral cavity. While most of these microorganisms are part of the normal oral microbiota, they can acquire pathogenic characteristics under certain conditions.

The aim of this research is to study different types of microorganisms that inhabit tooth structures and assess the antibacterial properties of temporary filling materials.

A series of related articles, publications, and results of different studies have been analyzed and summarized in order to provide an overview of the main pathogens affecting the hard dental tissues, the pulp, and the periodontium, as well as the antibacterial properties of temporary filling materials.

The literature review of the articles shows that many species affect the dental tissues, and different medications and strategies for their elimination are needed.

Furthermore, the review highlights that temporary filling materials have antimicrobial properties against certain microorganisms and have to be combined with different intracanal dressings.


microorganisms, microleakage, temporary filling materials

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Bae KS, Baumgartner JC, Shearer TR, David LL. Occurrence of Prevotella nigrescens and Prevotella nigrescens in infections of endodontic origin. J Endod. 1997;23:620–3

Bystrom A, Claesson R, Sundqvist G. The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals. Endod Dent Traumatol. 1985;1:170–5

Chávez de Paz Villanueva LE, Fusobacterium nucleatum in endodontic flare-ups. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002 Feb;93(2):179-83

Devika warrier E, Dr.Jayalakshmi, A Review on temporary restorative materials, IJPSR, Vol. 7 No. 7 Jul 2016

Dudeja PG, Dudeja KK, Srivastava D, Grover S. Microorganisms in periradicular tissues: Do they exist? A perennial controversy, J Oral Maxillofac Pathol. 2015 Sep-Dec; 19(3): 356–363.

Forssten SD, Björklund M, Ouwehand AC. Streptococcus mutans, Caries and Simulation Models, 2010, PMC, 2(3): 290–298.

Gomes BP, Lilley JD, Drucker BD. Associations of endodontic symptoms and signs with particular combinations of specific bacteria, Intern.Endod.Journal, Volume29, Issue2, March 1996, Pages 69-75

Gonçalvesn SNF, De Vasconcelos RA, Cavalcanti BN, Camargo CHR. Persistent infection by Staphylococcus epidermidis in endodontic flare-up: a case report, Gen Dent. 2016 Mar-Apr;64(2):e18-21

Haapasalo М. Bacteroides spp. in dental root canal infections, Endod Dent Traumatol, . 1989 Feb;5(1):1-10.

Karayasheva D, Radeva E. Importance of Enterococci (Enterococcus faecalis) for Dental Medicine - Microbiological Characterization, Prevalence and Resistance, July 2017, International Journal of Science and Research (IJSR) 6(7)

Karayasheva D, Radeva E. A survey done among dental students on their knowledge in endodontic microbiology. J of IMAB. 2020 Apr-Jun;26(2):3045-3052.

Kaushik N, Rehani U, Agarwal A, Kaushik M, Adlakha V. Antimicrobial Efficacy of Endodontic Irrigants against Enterococcus Faecalis and Escherichia Coli: An in vitro study, Int J Clin Pediatr Dent. 2013 Sep-Dec; 6(3): 178–182

Lamont RJ, Demuth DR, Davis CA, Malamud D, Rosan B. Salivary-Agglutinin-Mediated Adherence of Streptococcus-Mutans to Early Plaque Bacteria. Infect. Immun. 1991;59:3446–3450

MacFarlane TW, Helnarska S. The microbiology of angular cheilitis. Br Dent J 1976; 140: 403– 406

Machado de Oliveira JC , Siqueira Jr. JF, Alves GB, R Hirata Jr, A F Andrade, Detection of Porphyromonas endodontalis in infected root canals by 16S rRNA gene-directed polymerase chain reaction,J Endod. 2000 Dec;26(12):729-32.

Mahesh ACP, Sharada P, Chokshi K, Anupriya S, Ashwini BK. A correlative study of the levels of salivary Streptococcus mutans, lactobacilli and Actinomyces with dental caries experience in subjects with mixed and permanent dentition, J Oral Maxillofac Pathol. 2016 Jan-Apr; 20(1): 25–28

Marsh PD. Are dental diseases examples of ecological catastrophes? Microbiology-Sgm. 2003;149:279–294

Mayrand D, McBride BC, Edwards T, Jensen S. Characterization of Bacteroides asaccharolyticus and B. melaninogenicus oral isolates. Can J Microbiol 1980;26:1178.

McCormack MG, Smith AJ, Akram AN, Jackson M, Robertson D, Edwards G. Staphylococcus aureus and the oral cavity: An overlooked source of carriage and infection?, American Journal of Infection Control, Volume 43, Issue 1, 1 January 2015, Pages 35-37

Montagner F, Jacinto RC, Signoretti FGC, Gomes B. Treponema Species Detected in Infected Root Canals and Acute Apical Abscess Exudates, Journal of Endodontics, Volume 36, Issue 11, November 2010, Pages 1796-1799

Ohara-Nemoto Y, Haraga H, Kimura S, Nemoto TK, Occurrence of staphylococci in the oral cavities of healthy adults and nasal–oral trafficking of the bacteria, 2008, J. of medical microbiology,Volume 57, Issue 1

Pereira RS, Rodrigues V, Furtado WT, Gueiros S, Pereira GS, Avila-Campos MJ. Microbial analysis of root canal and periradicular lesion associated to teeth with endodontic failure, Anaerobe, Volume 48, December 2017, Pages 12-18

Radeva E., Indjov B., Vacheva R., In vitro study of the effectiveness of intracranial irritants on Candida Albicans. Journal of IMAB 2007, vol. 13, book 2

Radeva EN, Uzunov TT, Vacheva RS. In Vitro Study of the Antibacterial Activity of Calcium Hydroxide on Candida Albicans, International Journal of Science and Research, 2014, (IJSR) ISSN (Online): 2319-7064

Rajaram A, Kotrashetti VS, Somannavar PD, Ingalagi P, Bhat K. Culture-based identification of pigmented Porphyromonas and Prevotella species in primary endodontic infections, 2016, J Dent Res Dent Clin Dent Prospects. 10(3): 136–141

Rôças IN, Siqueira Jr. JF. Root canal microbiota of teeth with chronic apical periodontitis. J Clin Microbiol. 2008;46:3599–606

Singh А, Verma Р,Murari А, Agrawal А. Oral candidiasis: An overview, J Oral Maxillofac Pathol. 2014 Sep; 18(Suppl 1): S81–S85.

Siqueira Jr JF, Rôças IN. Microbiology and Treatment of Acute Apical Abscesses, Clin Microbiol Rev. 2013 Apr; 26(2): 255–273.

Slutzky H, Slutzky-Goldberg I, Weiss E I, Matalon S, Antibacterial properties of temporary filling materials, J Endod. 2006 Mar;32(3):214-7.

Smith AJ, Robertson D, Tang MK, Jackson MS, MacKenzie D, Bagg J, Staphylococcus aureus in the oral cavity: a three-year retrospective analysis of clinical laboratory data, British Dental Journal ,volume 195, pages 701–703 (2003)

Sundqvist G, Johansson E. Neutrophil chemotaxis induced by anaerobic bacteria isolated from necrotic dental pulps. Scand J Dent Res. 1980;88:113–21

Tanzer JM, Livingston J, Thompson AM. The microbiology of primary dental caries in humans. J. Dent. Educ. 2001;65:1028–1037

Wahjuningrum DA, Pramesti HD, Sihombing MR, Luthfiana Z, Devi, Roelianto M and Setyabudi, Chitosan Antibacterial Activity Against Streptococcus viridans, Mal J Med Health Sci 17(SUPP13): 54-59, Dec 2021

Wittgow Jr WC, Sabiston CB., Jr Microorganisms from pulpal chambers of intact teeth with necrotic pulps. J Endod. 1975;1:168–71

Xia Т, Baumgartner JC. Occurrence of Actinomyces in infections of endodontic origin, J Endod. 2003 Sep;29(9):549-52

Zaneva-Hristova D, Borisova-Papancheva T, Аntibacterial effect of irrigants and medications for temporary dressing on Enterococcus faecalis, Medinform, 2022, Issue 1



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