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Consolidation Of The Murburn Model Of Flagella-Aided Motility In Bacteria

Daniel Andrew Gideon, Pushparaj Annadurai, Kelath Murali Manoj

Abstract

Howard Berg had proposed a ‘rotary motor’ explanatory paradigm for the flagella-assisted motility of bacteria (1), claiming that proton motive force (pmf) provided the powering in such propulsion in low Reynold’s number regimes (2). Paul Boyer subsequently suggested a pmf-aided rotary functionalism for explaining Complex V’s role in photo-/oxidative- phosphorylations in chloroplasts/mitochondria. Michael Behe had questioned the ‘evolvability’ of such self-assembled multi-molecular protein complexes with rotary functions, calling these proposals ‘irreducibly complex’. Over the last 25 years, while championing a new stochastic cellular principle of life (complementing the deterministic principle of central dogma) called murburn concept, we discredited the idea of “pmf-assisted rotary functions” in cellular routines in the two systems above. Herein, we confirm some of our earlier predictions and provide further evidence/arguments supporting the murburn model of flagella-aided motility in bacteria: (i) the component proteins of C-ring and Mot proteins show large variations in monomeric constitution and amino acid sequences suggesting that they could not have evolved for rotary activity (3), (ii) the purported rotary fulcrum role of a single amino acid residue (MotB’s D/E32) is untenable as it is not conserved in a wide variety of bacteria surveyed, (iii) the C-ring proteins and cytoplasm-side of MotA have a relatively higher concentration of charged residues that enable DRS recruitment/utilization, and (iv) the basal module is a Type-3 secretory system ejecting fluid wherein MotAB complex enable efficient proton delivery for water formation and expulsion via the rod.

Keywords

murburn concept, bacterial flagella, proton motive force, biological rotary motor

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DOI: http://dx.doi.org/10.14748/bmr.v35.10340

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About The Authors

Daniel Andrew Gideon
Department of Biochemistry, St. Joseph’s University, Langford Road, Bengaluru, Karnataka, India

Pushparaj Annadurai
Department of Medicinal Botany and Pharmacognosy, Nandha Siddha Medical College and Hospital, Erode, TamilNadu, India

Kelath Murali Manoj
Satyamjayatu: The Science & Ethics Foundation, Snehatheeram, Shoranur-2 (PO), Palakkad District, Kerala, India School of Artificial Intelligence, Amrita Vishwa Vidyapeetham (Coimbatore) Amritanagar, Ettimadai, TamilNadu, India

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